黄方（教授）

»姓名： 黄方

»职称： 教授

系属： 生物工程与技术中心

»最高学位： 博士

»学科： 生物化工

»所学专业： 化学

»电子邮箱：fhuang@upc.edu.cn

»联系电话：0532-86981235

»地址邮编：山东省青岛市黄岛区长江西路66号365游戏大厅（华东）化学工程学院,266580

»个人主页：http://pme.cce.upc.edu.cn/

◎学习与工作经历

 ◎研究方向

蛋白质工程、酶工程、单分子荧光技术、荧光成像技术

◎承担科研课题

1）菌视紫红质去折叠态和折叠中间态的单分子研究，国家基金面上项目，2011年1月至2013年12月，主持。

2）多孔薄膜和溶液聚集体结构自组装、理论模拟与性能的基础研究，国家基金重点项目，2011年1月至2014年12月，参与

3）生物物理与单分子荧光研究，山东省杰出青年基金，2010年10月至2013年10月，主持

4）教育部新世纪优秀人才支持计划，2011年1月-2013年12月，主持

5）心血管疾病相关GPCR新药物靶点的基础研究，国家重点基础研究发展计划（973计划），2012年1月至 2016年12月，主持

6）单螺旋跨膜多肽在生物膜中取向的研究，国家自然科学基金面上项目，2013年1月-2016年12月，主持

7）用于重大恶性肿瘤早期预警的等离子体纳米镀膜检测芯片关键技术研究，2015年7月-2017年6月，主持

8）多态蛋白状态转变的单分子荧光研究，国家自然科学基金面上项目，2016年01月-2019年12月，主持

 ◎获奖情况

2012年，山东青年五四奖章

2019年，青岛市自然科学奖三等奖

 ◎代表性论文和专利

1Huang, H., Ge, B., Zhang, S., Li, J., Sun, C., Yue, T. & Huang, F. Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes. Materials2019, 12, 349.

2Huang, H., Ge, B., Sun, C., Zhang, S. & Huang, F. Membrane curvature affects the stability and folding kinetics of bacteriorhodopsin. Process Biochem2019, 76, 111-117.

3Wang, X., Wang, C., Xu, B., Wei, J., Xiao, Y. & Huang, F. Adsorption of intrinsically disordered barnacle adhesive proteins on silica surface. Appl Surf Sci2018, 427, 942-949.

4Wang, X., Chen, H., Lu, X., Chi, H., Li, S. & Huang, F. Probing the interaction mechanisms between transmembrane peptides and the chaperonin GroEL with fluorescence anisotropy. Spectrochim Acta A Mol Biomol Spectrosc2018, 194, 1-7.

5Song, Y., Ge, B., Lao, J., Wang, Z., Yang, B., Wang, X., He, H., Li, J. & Huang, F. Regulation of the Oligomeric Status of CCR3 with Binding Ligands Revealed by Single-Molecule Fluorescence Imaging. Biochemistry2018, 57, 852-860.

6Khan, N. U., He, H., Wang, X., Ge, B., Wang, Q., Liu, X., Lao, J., Wang, Y., Li, J., Wang, Z., Zhou, S. & Huang, F. A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells. Chem Commun2018, 54, 778-781.

7Khan, N. U., Feng, Z., He, H., Wang, Q., Liu, X., Li, S., Shi, X., Wang, X., Ge, B. & Huang, F. A facile plasmonic silver needle for fluorescence-enhanced detection of tumor markers. Anal. Chim. Acta2018, 1040, 120-127.

8Jacob, M. H., D'Souza, R. N., Schwarzlose, T., Wang, X., Huang, F., Haas, E. & Nau, W. M. Method-Unifying View of Loop-Formation Kinetics in Peptide and Protein Folding. J Phys Chem B2018, 122, 4445-4456.

9Huang, H., Yang, B., Ge, B., Lao, J., Zhou, S. & Huang, F. Using self-cleavable ternary fusion pattern for efficient preparation of Bacteriorhodopsin. Process Biochem2018, 64, 206-212.

10He, J., Xi, L., Sun, X., Ge, B., Liu, D., Han, Z., Pu, X. & Huang, F. Enhanced hydrogen production through co-cultivation of Chlamydomonas reinhardtii CC-503 and a facultative autotrophic sulfide-oxidizing bacterium under sulfurated conditions. Int J Hydrogen Energy2018, 43, 15005-15013.

11He, H., Wang, X. & Huang, F. in Membrane Biophysics   (eds Hongda Wang & Guohui Li)  147-173 (Springer, 2018).

12He, H., Li, S., Shi, X., Wang, X., Liu, X., Wang, Q., Guo, A., Ge, B., Khan, N. U. & Huang, F. Quantitative Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjug Chem2018, 29, 3658-3666.

13Chi, H., Xu, B., Liu, Z., Wei, J., Li, S., Ren, H., Xu, Y., Lu, X., Wang, X., Wang, X. & Huang, F. Combined thermodynamic and kinetic analysis of GroEL interacting with CXCR4 transmembrane peptides. Biochim. Biophys. Acta2018, 1862, 1576-1583.

14Zhang, H.-T., Zhao, Y.-Y., Ge, B.-S. & Huang, F. Study on The Interaction of Metamorphic Protein Mad2 and Its Ligand Cdc20(121-138). Progress in Biochemistry and Biophysics2017, 44, 232-241.

15Wang, X., Xiao, Y., Wang, C., Niu, J., Ma, J., Wang, H., Li, X., Chen, X. & Huang, F. Hierarchical interfacial structuring of the calcareous base of the barnacle Balanus albicostatus over different length scales. Mater Chem Phys2017, 192, 48-57.

16Wang, X., Wang, Y., He, H., Ma, X., Chen, Q., Zhang, S., Ge, B., Wang, S., Nau, W. M. & Huang, F. Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells. ACS Appl Mater Interfaces2017, 9, 17799-17806.

17Wang, X., Wang, C., Pan, M., Wei, J., Jiang, F., Lu, R., Liu, X., Huang, Y. & Huang, F. Chaperonin-Nanocaged Hemin as an Artificial Metalloenzyme for Oxidation Catalysis. ACS Appl Mater Interfaces2017, 9, 25387-25396.

18Lao, J., He, H., Wang, X., Wang, Z., Song, Y., Yang, B., Ullahkhan, N., Ge, B. & Huang, F. Single-Molecule Imaging Demonstrates Ligand Regulation of the Oligomeric Status of CXCR4 in Living Cells. J Phys Chem B2017, 121, 1466-1474.

19He, H., Liu, X., Li, S., Wang, X., Wang, Q., Li, J., Wang, J., Ren, H., Ge, B., Wang, S., Zhang, X. & Huang, F. High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots. Anal. Chem.2017, 89, 11831-11838.

20Ge, B., Lao, J., Li, J., Chen, Y., Song, Y. & Huang, F. Single-molecule imaging reveals dimerization/oligomerization of CXCR4 on plasma membrane closely related to its function. Sci Rep2017, 7, 16873.

21Zhao, Y., Li, L., Wu, C., Jiang, X., Ge, B., Ren, H. & Huang, F. Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state. Protein Eng Des Sel2016, 29, 23-29.

22Wang, X., Wang, Y., He, H., Chen, X., Sun, X., Sun, Y., Zhou, G., Xu, H. & Huang, F. Steering graphene quantum dots in living cells: lighting up the nucleolus. J Mater Chem B2016, 4, 779-784.

23Wang, X., He, H., Wang, Y., Wang, J., Sun, X., Xu, H., Nau, W. M., Zhang, X. & Huang, F. Active tumor-targeting luminescent gold clusters with efficient urinary excretion. Chem Commun2016, 52, 9232-9235.

24He, H., Wang, Z., Cheng, T., Liu, X., Wang, X., Wang, J., Ren, H., Sun, Y., Song, Y., Yang, J., Xia, Y., Wang, S., Zhang, X. & Huang, F. Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Appl Mater Interfaces2016, 8, 28529-28537.

25He, H., Wang, X., Cheng, T., Xia, Y., Lao, J., Ge, B., Ren, H., Khan, N. U. & Huang, F. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chem--Eur J2016, 22, 5863-5867.

26Chi, H., Wang, X., Li, J., Ren, H. & Huang, F. Chaperonin-enhanced Escherichia coli cell-free expression of functional CXCR4. J Biotechnol2016, 231, 193-200.

27Yu, D., Wang, M., Zhu, G., Ge, B., Liu, S. & Huang, F. Enhanced photocurrent production by bio-dyes of photosynthetic macromolecules on designed TiO2 film. Sci Rep2015, 5, 9375.

28Wang, X., Sun, X., He, H., Yang, H., Lao, J., Song, Y., Xia, Y., Xu, H., Zhang, X. & Huang, F. A two-component active targeting theranostic agent based on graphene quantum dots. J. Mater. Chem. B2015, 3, 3583--3590.

29Wang, X., Huang, H., Sun, C. & Huang, F. Structural analysis of bacteriorhodopsin solubilized by lipid-like phosphocholine biosurfactants with varying micelle concentrations. J Colloid Interface Sci2015, 437, 170-180.

30Ge, B., Wang, M., Li, J., Liu, J. & Huang, F. Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli. Process Biochem2015, 50, 285-293.

31Chi, H., Wang, X., Li, J., Ren, H. & Huang, F. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES. Sci Rep2015, 5, 17037.

32Wang, X., Sun, X., Lao, J., He, H., Cheng, T., Wang, M., Wang, S. & Huang, F. Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery. Colloids Surf. B2014, 122, 638-644.

33Song, B., Sun, Q., Li, H., Ge, B., Pan, J. S., Wee, A. T. S., Zhang, Y., Huang, S., Zhou, R., Gao, X., Huang, F. & Fang, H. Irreversible Denaturation of Proteins through Aluminum-Induced Formation of Backbone Ring Structures. Angew Chem, Int Ed2014, 53, 6358-6363.

34Huang, F., Johnson, C. M., Petrovich, M. & Fersht, A. R. Don't waste good methods on bad buffers and ambiguous data. Proc Natl Acad Sci U S A2013, 110, E331-332.

35Leith, J. S., Tafvizi, A., Huang, F., Uspal, W. E., Doyle, P. S., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. Sequence-dependent sliding kinetics of p53. Proc Natl Acad Sci U S A2012, 109, 16552-16557.

36Tafvizi, A., Huang, F., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. A single-molecule characterization of p53 search on DNA. Proc Natl Acad Sci U S A2011, 108, 563-568.

37Rajagopalan, S., Huang, F. & Fersht, A. R. Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53. Nucleic Acids Res.2011, 39, 2294-2303.

38Huang, F., Ying, L. & Fersht, A. R. Direct observation of barrier-limited folding of BBL by single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA2009, 106, 16239-16244.

39Huang, F., Rajagopalan, S., Settanni, G., Marsh, R. J., Armoogum, D. A., Nicolaou, N., Bain, A. J., Lerner, E., Haas, E., Ying, L. & Fersht, A. R. Multiple conformations of full-length p53 detected with single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA2009, 106, 20758-20763.

40Huang, F., Lerner, E., Sato, S., Amir, D., Haas, E. & Fersht, A. R. Time-resolved fluorescence resonance energy transfer study shows a compact denatured state of the B domain of protein A. Biochemistry2009, 48, 3468-3476.

41Tafvizi, A., Huang, F., Leith, J. S., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. Tumor suppressor p53 slides on DNA with low friction and high stability. Biophys. J.2008, 95, L01-L03.

42Huang, F., Sato, S., Sharpe, T. D., Ying, L. & Fersht, A. R. Distinguishing between cooperative and unimodal downhill protein folding. Proc Natl Acad Sci USA2007, 104, 123-127.

43Huang, F., Hudgins, R. R. & Nau, W. M. Primary and secondary structure dependence of peptide flexibility assessed by fluorescence-based measurement of end-to-end collision rates. J Am Chem Soc2004, 126, 16665-16675.

44Huang, F. & Nau, W. M. A conformational flexibility scale for amino acids in peptides. Angew Chem Int Ed2003, 42, 2269-2272.

 ◎学术兼职

中国化学会生物物理化学专业委员会委员(2012.1-)，副主任（2018.07-）

中国生物物理学会单分子生物学分会理事(2015.07-)

山东省物理学会生物物理专业委员会副主任(2015.12-)

◎指导研究生情况

自2009年以来已指导10余名博士研究生，20多名硕士研究生，其中多人曾获国家奖学金，多人毕业后出国或者在读期间到国外参加联合培养。

 ◎荣誉称号

山东省杰出青年基金获得者

教育部新世纪优秀人才人选者